Paulo Cezar NovaisUNIMAR University, Brazil
Title: Down-regulation of miR-133b and miR-221 in patients with breast cancer and brain metastasis
The microRNAs (miRNAs) are short non-coding RNAs that regulate gene expression. The expression of microRNAs is not fully known in brain metastasis mechanism, but there are evidences that they are important in the development of brain and breast tumors. The aim of our study was to measure the expression of the microRNAs: miR-221, miR-133b and miR-126 and the genes: MGMT, XIAP and IDH2.We utilized for this study brain metastasis samples from patients diagnosed with breast cancer. For the analysis of the samples we used the quantitative polymerase chain reaction (qPCR) for gene expression quantification of the genes (MGMT, XIAP and IDH2) and microRNAs (miR-221, miR-133b and miR-126). The miR-133b and miR-221 were significantly down-regulated in the brain metastasis and breast cancer samples. All three genes didn’t have any statistically significant differences. However, MGMT and IDH2 was up-regulated in brain metastasis, and XIAP was up-regulated in breast cancer. The miR-133b and miR-221 was down-regulated in the brain metastasis and breast cancer samples.
Paulo Cezar Novais, 55 years old, holds a master's and doctorate in Medical Sciences from FMRP-USP - Faculty of Medicine of the University of São Paulo, is a professor and researcher at UNIMAR University of Marília and a collaborating researcher at FMRP-USP. It has 40 published complete articles, has H-index-8, 24 publications in web of Sciense, 132 citing articles. Orients students in the Interdisciplinary Master's Program in Structural and Functional Interactions in Rehabilitation at UNIMAR.