Selman celik
Yeditepe University, TurkeyPresentation Title:
An implantable rutin incorporated PCL/collagen electrospun nanofibers for effective growth inhibition of lung cancer cells: Possible application in prevention of lung cancer local recurrence
Abstract
Background: Lung cancer remains a global challenge, and further chemopreventive therapies are still immediately required. Emerging evidence has revealed the potent anti-cancer effects of Rutin. Thus, to explore an efficient chemopreventive strategy for lung cancer, the antiproliferative effects of Rutin and Rutin- PCL/collagen nanofibers against breast cancer cells were assessed.
Methods: Rutin incorporated electrospun nanofibers were successfully fabricated via electrospinning and characterized through Dynamic Light Scattering (DLS), Fourier-transform infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FE-SEM). Anti-proliferative and apoptotic effects of rutin- PCL/collagen nanofibers were evaluated using MTT and flow-cytometric assays, respectively. Also, real-time polymerase chain reaction (Real-Time PCR) was used to determine the gene expression levels of apoptotic genes.
Results: The results showed that that electrospinning of 10% (w/v) PCL- collagen blend produced a bead-free electrospun meshes with average diameter in the range of 300–400nm. Also, loading of Rutin into the nanofibers improved mechanical properties than without drug-loaded NFs. MTT assay showed that loading of Rutin into PCL/collagen nanofiber enhanced noticeably cytotoxicity in the human lung cancer cells. This result was associated with alteration the mRNA levels of bax, bcl-2, p53, Cyclin D1, caspase-3, caspase-7, and hTERT genes.
Conclusion: In conclusion, the drug-loaded nanofibers showed an excellent capacity to inhibit A549 lung cancer cells in vitro than the free Rutin. Therefore, the fabricated drug-encapsulated NFs may achieve a safe and suitable application for lung cancer relapse rate after surgery.
Keywords: PCL- collagen, Nanofiber, Rutin, Lung cancer, Apoptotic gene
Biography
Selman Celik has been working as an academician at Yeditepe University's faculty of health sciences since 2016. She completed her undergraduate and graduate degrees in nursing at Yeditepe University. She completed his doctorate in internal medicine at Marmara University. She teaches internal medicine nursing, general pathology, oncology nursing, and health tourism courses at Yeditepe University. She mainly focuses on research in the fields of lung health and lung diseases, especially COPD management. She is an active member of many institutions and organisations related to his field of work.
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